Acellular Lung Scaffolds Direct Differentiation Of Endoderm ~ image credit
Primary Airway Epithelial Cell Culture From Lung Transplant ~ image credit
Role Of Airway Epithelial Barrier Dysfunction In ~ image credit
Normal Human Small Airway Epithelial Cells Primary ~ image credit
The Role Of Respiratory Epithelium In Host Defence Against ~ image credit
Notch3 Contributes To Rhinovirus Induced Goblet Cell ~ image credit
Lung airway epithelial cells. Immunohistochemistry of lungs from mice subjected to the dra asthma model showed a predominant expression of csf1 in airway epithelial cells aecs indicating that aecs are the major site of csf1 production in lung figure 1e. Airway epithelial cells aecs are located at the interface between the external and internal milieu and are exposed to an array of inhaled gases and particles on a daily basis. Lung and airway epithelial cells generated from human pluripotent stem cells either embryonic stem cells escs or induced pluripotent stem cells ipscs would have multiple applications including recel lularization of decellularized lung scaffolds to provide an autologous graft for transplantation study of human lung development modeling. These tall slender cells are anchored on the base of the epithelium which is one layer thick. The type ii alveolar epithelial cells were capable of surfactant protein b uptake and stimulated surfactant release providing evidence of specific function. For example these basal cells respond to injury of the airway epithelium migrating to cover a site denuded of differentiated epithelial cells and subsequently differentiating to restore a healthy epithelial cell layer.
Furthermore lung development research has resulted in new tools to study epithelial cell function in inflammatory lung disease. Indeed recently the ability to generate aecs from human embryonic stem cells esc and induced pluripotent stem cells ipsc 3 5 has contributed insights into the development of the airway epithelium and will likely continue to do so. Thus as the air sacs of the lungs are approached the respiratory epithelium is formed primarily of cuboidal epithelial cells. Certain parts of the respiratory tract such as the oropharynx are also subject to the abrasive swallowing of food. The ability to generate lung and airway epithelial cells from human pluripotent stem cells hpscs would have applications in regenerative medicine modeling of lung disease drug screening and. Bronchialtracheal epithelial cells large airway lobar epithelial cells and lung small airway epithelial cells are cultured in bronchialife tm medium.
Lifeline lung cells are cryopreserved at the end of primary or secondary culture to ensure the highest viability and plating efficiency.
Airway Cells Lonza ~ image credit
A Total Rnas Were Extracted From Primary Small Airways ~ image credit
Air Liquid Interface Culture Of Human And Mouse Airway ~ image credit